Molecular diagnosis of hereditary colorectal cancers

Molecular Diagnosis of Hereditary Nonpolyposis Colorectal Cancer (hnpcc)

iii Imagine a single cell in your body, turning against you, becoming malignant, and eventually causing a life-threatening disease. Cancer is both fascinating and horrifying. For decades, scientists have battled to unravel the underlying mechanisms. Although significant progress has been made, the heart of the mystery remains unsolved.

Genetic Basis of Hereditary Colorectal Cancers: Hereditary Nonpolyposis Colorectal Cancer and Familial Adenomatous Polyposis

Molecular pathogenesis of sporadic colorectal cancers

Colorectal cancer (CRC) results from the progressive accumulation of genetic and epigenetic alterations that lead to the transformation of normal colonic mucosa to adenocarcinoma. Approximately 75% of CRCs are sporadic and occur in people without genetic predisposition or family history of CRC. During the past two decades, sporadic CRCs were classified into three major groups according to frequ...

Hyperplastic Polyps Are Innocuous Lesions in Hereditary Nonpolyposis Colorectal Cancers

Aims. To compare methylation profiles, protein expression, and microsatellite instability (MSI) of sporadic, HNPCC, and familial hyperplastic polyps (HPs). Methods. Methylation-specific PCR (MSP) and pyrosequencing assessed p16, MGMT, hMLH-1, MINT 1, and MINT 31 methylation. IHC (Immunohistochemistry) assessed Ki67, CK20, hMLH-1, hMSH-2, and hMSH-6 protein expression. MSI analysis was performed...

Gene assembling: a new approach in molecular diagnosis of hereditary breast cancer

 Abstract Background: Many disease susceptibility genes are large and consist of many exons in which point mutations are scattered throughout. Scanning each exon individually represents a tedious task which can be time consuming and expensive. There has been increasing demand for rapid and accurate methods for full scanning of unknown point mutations in large multi-exon genes. Gene Assembling i...